Tris hcl indicator forex. Cartilage (15 mg wet weight) was freeze-milled in a Biopulverizer (Biospec Products, Bartlesville, OK) and the powder was extracted for 20 h at 4°C in three volumes (μl/mg wet weight) of (1) 50 mM Tris–HCl, pH ; (2) 50 mM Tris–HCl, mM NaCl, pH ; (3) 50 mM Tris–HCl, mM NaCl, % Nonidet P, pH ;.

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